In contrast, fluorescent thermometry (a specific application of the more general laser-induced fluorescence (LIF) technique) represents one of the most accurate temperature measurement techniques at the macroscale. In this technique, a fluorescent dye whose fluorescence intensity is a strong function of temperature is dissolved in a fluid of …
Get a quoteA fluorometer is an instrument designed to measure the various parameters of fluorescence, including its intensity and wavelength distribution of the emission after excitation. Chemists use this to identify properties and the amount of specific molecules in a sample. Chemists call molecules that can exhibit fluorescence fluorophores.
Get a quoteThere are three main categories of TES technologies: sensible heat storage (which stores energy proportionally to the temperature change of a medium), latent heat storage (with thermal energy stored through a phase …
Get a quoteA molecule that is excited can return to the ground state by several combinations of mechanical steps that will be described below and shown in Figure 9.5.2 9.5. 2 .The deactivation process of fluorescence and phosphorescence involve an emission of a photon radiation as shown by the straight arrow in Figure 9.5.2 9.5. 2.
Get a quoteCold vapor atomic fluorescence spectroscopy (CVAFS) uses the same technique as AFS, but the preparation of the sample is adapted specifically to quantify the presence of heavy metals that are …
Get a quoteThin film deposited chromium-doped aluminium oxide (Cr–Al 2 O 3, ruby) thermographic phosphors for temperature measurement have been used. 64 Zinc silicate (Zn 2 SiO 4:Mn 2+) has been shown to be highly suitable for fluorescence intensity ratio temperature measurement over the range of 30 to 300 °C with a sensitivity of 12.2%. 65 …
Get a quoteIn view of the uncertainty in the calibration process of two-color plane laser-induced fluorescence (PLIF) temperature measurement, a new calibration method is proposed, in which the influence of fluorescence yield is considered. The calibration process was carried out at high and low temperature region, respectively. Then, the bias …
Get a quoteThermal energy storage (TES) systems store heat or cold for later use and are classified into sensible heat storage, latent heat storage, and thermochemical heat storage. Sensible heat storage systems raise the temperature of a material to store heat.
Get a quoteThe basis of chlorophyll fluorescence measurements. The principle underlying chlorophyll fluorescence analysis is relatively straightforward. Light energy absorbed by chlorophyll molecules in a leaf can undergo one of three fates: it can be used to drive photosynthesis (photochemistry), excess energy can be dissipated as heat or it can …
Get a quotecorrespond to thermally coupled energy levels to determine temperature. The FIR method is independent of spectrum losses and fluctuations in the excitation intensity. However, t. he narrow energy gap of the thermally coupled energy level s leads to low temperature measurement sensitivity. In order to improve the temperature measurement sensitivity,
Get a quoteCao-Paz et al. [ 40] demonstrated a multi-point sensor based on U-shape fibre to measure the electrolyte density in lead-acid batteries in 2010. The multi-point sensor used in the study consisted of 5 optical fibres which were placed on the U-shape mould with a radius length of 1.5 mm, as shown in Fig. 23 (a).
Get a quoteThe fluorescence signal would linearly be proportional to the laser input irradiance. However, A 21 is usually less than Q 21; the fluorescence efficiency would be less than 1, which diminishes the sensitivity of LIF; Q 21 must be evaluated if LIF is used to perform quantitative measurement. Actually, the evaluation of Q 21 is not practical since …
Get a quoteThe temperature regulation is also important during the fluorescence measurements as it could cause a 1–2% of the reduction in fluorescence intensity per degree of temperature rise. If the investigated material is photosensitive, it can be degraded into different fluorescent products giving rise to different fluorescence lifetimes.
Get a quoteThe principle of FRET relies on the transfer of excitation energy of a donor fluorophore to a nearby acceptor fluorophore in a non-radiative fashion through long-range dipole-dipole interactions, when the distance separating them is 8 to 10 nanometers or less. For protein interaction studies, the donor and acceptor fluorophores are attached to ...
Get a quoteThe Florida State University, Tallahassee, Florida, 32310. The technique of fluorescence resonance energy transfer (more commonly referred to by the acronym FRET), when applied to optical microscopy, permits determination of the approach between two molecules within several nanometers, a distance sufficiently close for molecular interactions to ...
Get a quotePrinciple. Infrared (IR) thermography is a non-intrusive, two-dimensional technique for the whole-field measurement of surface temperature. An infrared scanning radiometer (IRSR) detects the electromagnetic energy radiated in the IR spectral band by the surface of interest and converts it into an electronic signal.
Get a quoteThe energy, E, carried by any one quantum is proportional to its frequency of oscillation, that is E = hν = hc ergs λ where ν is the frequency, λ the related wavelength and h = Planck''s constant (6.624 x 10-27 ergs/seconds). The energy of a single quantum is too small for convenience and it is usual to
Get a quoteQuantum dots (QD), semiconductor nanoparticles that emit fluorescence, have been applied to measure the temperature in living cells (Fig. 2e . The luminescence properties of QDs undergo temperature-dependent optical changes, such as a red-shift of the photoluminescence peak and decrease of the fluorescence intensity upon heating.
Get a quoteThe energy storage consists of the cabinet itself, the battery for energy storage, the BMSS to control the batteries, the panel, and the air conditioning to maintain the battery …
Get a quoteThe method consists of using optical fiber sensors to monitor fluorescence from a fluorescent dye that has been doped into the processed resin. Temperature is …
Get a quoteFluorescence and Phosphorescence occur at wavelengths that are longer than their absorption wavelengths.Phosphorescence bands are found at a longer wavelength …
Get a quoteIt discusses two specific measurement applications to illustrate the utility of thermographic phosphors (TPs) in various scenarios where standard temperature …
Get a quoteThe temperature-sensitive nature of molecular fluoresence provides the basis for designing optical detection systems whereby changes in fluorescent intensity, peak position, or …
Get a quoteIn this paper, we address several experimental design issues: (a) the supportive role of fluorescence anisotropy measurements to the measuring concept, (b) the experimental …
Get a quoteMeasurements and analysis show that the combination of QD fluorescence thermometry and axial localization of DH-PSF enables the acquisition of thermal image information in intracellular 3D microregions. Using this system, temperature variations at 3D locations in living cells exposed to ionomycin Ca 2+ stress were also …
Get a quoteFluorescence-based sensors, incorporated in optical fibers, are based on the principle of operation of fluorescence lifetime measurement [42,46]. There was a considerable research effort on fluorescence-based thermometry during the 1990s, during which the principle of fluorescence decay of phosphor materials was implemented in …
Get a quoteThe double-filled PAN composite fibers also had a good energy storage density, reaching an energy storage density of 44.02 mJ/cm 3 under the action of an electric field of 420 kV/cm 3, which was 1.64 times of the energy storage density of pure PAN (26.84 mJ/cm 3). More importantly, the dual-filler PAN composite fibers also had a …
Get a quoteThere are different types of fluorescence measurements used under different type of study and the suitability of the photosynthetic system. The analysis of Chl fluorescence intensity, peak, bandwidth, etc., gives an insight to the structure and function of the photosynthetic system, energy transducing and the pigment protein architecture in …
Get a quoteApplication of single-photon absorption laser-induced fluorescence (LIF) spectroscopy for non-intrusive measurement of neutral xenon and singly charged xenon ion kinetic temperatures in the discharge chamber of a gridded radiofrequency ion source is demonstrated. A LIF spectrum analysis approach including hyperfine structure …
Get a quoteThe chapter outlines general principles of fluorescence spectroscopy. Basic principles of radiative and nonradiative transitions (including the Jablonski diagram and Franck–Condon principle) are described and explained. The fundamentals of important fluorescence techniques, such as the steady-state and time-resolved measurements, …
Get a quoteAssuming each collision of F and Q results in quenching of F (γ = 1), using 65°C as a reasonable temperature for RT-PCR fluorescence measurements, and substituting 0.4335 cp for the viscosity of water, and 4.8 and 5.9 Å for the radii of fluorescein and tetramethylrhodamine, respectively, k q is found to equal 4.37 × 10 9 M −1 s −1.
Get a quoteDelepine and Jensen realized the distributed temperature measurement of an underground nuclear waste storage and the nuclear reactor cooling circuit, respectively. The DFOS wild application in various fields under different tough conditions exhibits its mature and stable performance and shows great potential in solving the power …
Get a quoteFluorescence is an instantaneous phenomenon that begins as soon as the light is absorbed and ends as soon as the incident light is cut off. The absorbed energy may be released in 10-6 seconds or less, but in stages, resulting in radiations of varying frequency. Fluorescence is a secondary phenomenon that occurs as a result of the …
Get a quoteFluorescence. Fluorescence spectrophotometry is based on fluorescence, which is a photoluminescence event (photo = light; luminescence = the emission of light). In simple terms, it is the emission of light because of exposure to (and resultant absorption of) light. Here, this exposure to and absorption of light is called excitation.
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